Chlordanes were remarkably high in eggs from the United States (2500 +/- 1300 ng/g lw). The OCP profile in all countries was largely dominated by p,p’-DDE. In general, the worldwide trends

we observed in starling eggs were in accordance with the literature on human and environmental OHC data, which suggests that there is potential for using starling eggs as a biomonitoring tool on a large geographical scale. (C) 2012 Elsevier Ltd. All rights reserved.”
“Satellite-based remote sensing provides a unique opportunity to S63845 monitor air quality from space at global, continental, national and regional scales. Most current research focused on developing empirical models using ground measurements of the ambient particulate. However, the application of satellite-based exposure assessment in environmental health is still limited, especially for acute effects, because the development of satellite PM2.5 model depends on the availability of ground measurements. We tested the hypothesis that MODIS AOD (aerosol

optical depth) exposure estimates, obtained from NASA satellites, are directly associated with daily health outcomes. Three independent healthcare databases were used: unscheduled outpatient visits, hospital admissions, and mortality collected in Beijing metropolitan area, China during 2006. We use generalized linear models to compare the short-term effects of air pollution assessed

by ground CP-456773 mouse monitoring (PM10) with adjustment of absolute humidity (AH) and AH-calibrated AOD. Across all databases we found that both AH-calibrated AOD and PM10 (adjusted by AH) were consistently associated with elevated daily events on the current day and/or lag days for cardiovascular diseases, ischemic heart diseases, and https://www.selleckchem.com/products/srt2104-gsk2245840.html COPD. The relative risks estimated by AH-calibrated AOD and PM10 (adjusted by AH) were similar. Additionally, compared to ground PM10, we found that AH-calibrated AOD had narrower confidence intervals for all models and was more robust in estimating the current day and lag day effects. Our preliminary findings suggested that, with proper adjustment of meteorological factors, satellite AOD can be used directly to estimate the acute health impacts of ambient particles without prior calibrating to the sparse ground monitoring networks. (C) 2012 Elsevier Ltd. All rights reserved.”
“This study investigated the occurrence of polychlorinated biphenyls (PCBs), and several additive brominated flame retardants (BFRs) in indoor dust and air from two Vietnamese informal e-waste recycling sites (EWRSs) and an urban site in order to assess the relevance of these media for human exposure.

Methods: Twenty patients on HD (aged 53.5 +/- 12.9 years) and 18 healthy individuals (aged 54.1 +/- 10.2 years) underwent a maximal and a submaximal cardiopulmonary test (CPETmax and CPETsubmax). click here Heart rate recovery (HRrec) 1 minute after exercise and time for VO2 to decrease by half (T1/2VO2) were determined. All subjects also completed 3 questionnaires: (a) the Beck Depression Inventory (BDI), (b) Quality of Life Index (QLI)-Spitzer Index and (c) SF-36 physical and mental component summary scales.

Results: HRrec after maximal (p=0.029) and submaximal test (p=0.041) was found to be lower in patients compared with healthy individuals. T1/2VO2 was raised by 29% (p=0.003) in patients

compared with controls. Moreover, a significantly higher BDI (by 133.7%), lower SF-36 physical (by 47.8%) and mental (by 42.9%) component summary score and lower QLI (by 32.1%) results were found in HD patients compared with controls. BDI (p=0.045), QLI (P=0.011), SF-36 physical (p=0.017) and mental component scales (p=0.021) were independently associated with HRrec in maximal tests in patients. Similar correlations remained for submaximal tests among HRrec. and Doramapimod research buy BDI (p=0.004), QLI (p=0.006), SF-36 physical (p=0.048) but not mental scales (p=0.369) in the patients’ group. T1/2VO2 also correlated to BDI (p=0.019), QLI (p=0.005) and SF-36 mental scale (p=0.017) in

maximal tests in these patients. In contrast, there was a correlation between HRrec and

BDI (p=0.004) in the control group for maximal tests only.

Conclusions: In conclusion, in HD patients, recovery indices following maximal and submaximal exercise tests were shown to provide useful indications of the patients’ psychological and quality-of-life profiles.”
“P>Background:

The relative effectiveness of GlideScope (R) videolaryngoscopy (GV) for nasotracheal intubation in pediatric patients is unclear. The purpose of this study SIS3 is to evaluate the usefulness of GV for nasotracheal intubation compared with direct laryngoscopy (DL) in pediatric patients.

Methods:

Our patient cohort consisted of 80 children < 10 years of age who required nasotracheal intubation for elective dental or facial surgery. The patients were randomly allocated to GV (n = 40) or DL (n = 40) group. The time to intubation (TTI), glottic view grade, frequency of Magill forceps use, and degree of difficulty in intubation were evaluated.

Results:

The median TTI was similar between the groups. The TTI of the former 20 patients was faster in the DL group (53.3 s, interquartile range: 42.0-64.3) than GV group (65.9 s, interquartile range: 56.0-93.9) (P = 0.007), whereas the TTI of the latter 20 patients was comparable between the groups. There were no significant differences in glottic view grade, frequency of Magill forceps use, and degree of difficulty in intubation between the two groups.

“
“BACKGROUND: Lung transplantation (LTx) is established as a life-saving treatment in end-stage ��-catenin signaling lung disease. However, long-term survival is hampered by the development of chronic rejection, almost synonymous with bronchiolitis

obliterans syndrome (BOS). The rejection is characterized by deposition of extracellular matrix in small airways. Fibroblasts/myofibroblasts are the main producers of extracellular matrix molecules such as proteoglycans. This study compared fibroblast phenotype and activity in the wound healing process at different points after LTx in patients who later did, or did not, develop BOS.

METHODS: Distally derived fibroblasts from patients 6 and 12 months after LTx and from healthy controls were analyzed for production of the proteoglycans versican, perlecan, biglycan, and decorin, with and without transforming growth factor (TGF)-beta(1). Fibroblast migration and proliferation were also studied.

RESULTS: At 6 and 12 months after LTx, versican production was higher in fibroblasts from LTx patients (p < 0.01 p < 0.01) than from controls. Fibroblasts from patients who later developed BOS were more responsive to TGF-beta(1)-induced synthesis of versican

and biglycan than patients without signs of rejection (p < 0.05). Production of perlecan and decorin was negatively correlated with fibroblast proliferation in fibroblasts at 6 months this website after LTx. In a more detailed case study of 2 patients, one with and one without BOS, the altered proteoglycan profile was associated with impaired lung function.

CONCLUSIONS: LTx changes the phenotype of fibroblasts to a non-proliferative but extracellular

matrix producing cell due to wound healing involving TGE-beta(1). If not controlled, this may lead to development of BOS. J Heart Lung Transplant 2011;30:945-54 (C) 2011 International Society for Heart and Lung Transplantation. All rights reserved.”
“The DLTS signal induced by a back contact barrier is studied both theoretically and through experiments on model circuits. A nonideal back contact is modeled either by a resistor and a capacitor, or by a germanium diode inversely polarized Belinostat in vivo with respect to the junction diode. Depending on the back contact properties, this may result in a positive or negative capacitance transient. For these model circuits the capacitance transient time constants and amplitudes are studied as a function of voltage pulse height and compared with signals originating from emission and slow capture from a defect level. These two origins of DLTS signals present very different properties, which opens possibilities to distinguish between them. (C) 2011 American Institute of Physics. [doi: 10.1063/1.

Thrombin activation of the platelet-rich plasma eliminated such stimulatory effects. In vivo, the platelet-rich plasma stimulated chondrogenesis on Day 14 and osteogenesis on Days 28 and 56, whereas thrombin-activated platelet-rich plasma acted as an inhibitor of such events. In addition, inflammatory cells were detected in demineralized bone matrix samples that were mixed with thrombin-activated platelet-rich plasma. These cells were not present in matrix mixed with platelet-rich plasma alone.

Conclusions: Platelet-rich plasma significantly increased in vivo demineralized bone matrix osteoinductivity only when used

without thrombin activation.”
“SETTING: Pulmonary tuberculosis (TB) patients reported in North Carolina (NC), USA, PHA-848125 purchase from 1996 to 2008 (inclusive).

OBJECTIVE: To compare prevalence of cavitary TB as a surrogate marker for advanced disease in low-caseload counties with high-caseload counties.

DESIGN: A multivariate log binomial regression model was used to estimate prevalence ratios (PRs) for cavitary TB.

RESULTS: The proportion of TB cases in low-caseload

counties vs. the total Mocetinostat order number of TB cases in NC over the study period increased from 10% in 1996 to 20% in 2008. After adjusting for human immunodeficiency virus (HIV) status, excess alcohol use and report year, patients in rural areas of low-caseload counties had greater prevalence (PR 1.40, 95%CI 1.19-1.64) of cavitary disease compared with patients from rural areas of high-caseload counties. The prevalence of cavitary TB did not differ between urban residents of high- or low-caseload ACY-738 in vitro counties (PR 1.00, 95%CI 0.86-1.16) after adjusting for HIV status, excess alcohol use and report year.

DISCUSSION: TB patients in rural areas of low-caseload counties presented with more advanced TB disease compared with patients from urban and/or high-caseload counties. Barriers to timely recognition of TB in rural low-caseload settings must be considered in TB control programs.”
“We describe a 50-year-old

woman with rapidly progressive pulmonary Mycobacterium abscessus (M. abscessus) infection accompanied by pleural effusion and organizing pneumonia (OP). CT scan showed consolidation, centrilobular shadows, ground-glass opacity (GGO), and cavities. A transbronchial lung biopsy showed nonnecrotizing granuloma surrounded by infiltrative lymphocyte-dominant inflammatory cells, and lymphocytes in bronchoalveolar lavage fluid (BALF) were increased. We considered OP occurred secondary to M. abscessus infection because clarithromycin, amikacin, and imipenem/cilastatin administration resulted in partial improvement. We added corticosteroids to the regimen, which resulted in a remarkable improvement. We report a case of pulmonary M. abscessus infection involving pleural effusion that responded favorably to medical therapy including corticosteroids.

Endothelial dysfunction was observed in both the aorta and femoral artery of the ovariectomy group but not of the control group. Increased marrow fat area was seen in the ovariectomy group (52.9% vs 21.6%; P < .01) owing to an

increase in fat cell number. Decreased erythropoetic marrow area (32.5% vs 48.6%; P < .05) was also observed in the ovariectomy group.

Conclusion: Reduced bone perfusion occurs in www.selleckchem.com/products/sbe-b-cd.html synchrony with reduced BMD. The most likely causes of reduced bone perfusion are a reduction in the amount of erythropoetic marrow and endothelial dysfunction after ovariectomy. (C) RSNA, 2010″
“Objectives: To evaluate registered nurse compliance with standard precautions and to analyze the factors that affect compliance.

Methods: Study data

were collected over a 3-month period from June to September 2007. The survey research method was used. A total of 1500 randomly sampled registered nurses from 18 hospitals in Hunan, China completed self-report questionnaires.

Results: Of the 1500 nurses included in the study, 1444 returned valid self-report questionnaires. Compliance with standard precautions was found to be low in the surveyed nurses. With a maximum possible score of 80, the quartile range of the overall score for compliance for all nurses was 48.29 (upper quartile score 76.36, lower quartile score 28.07), which was occupied by 64.7% of the participants. The factors most affecting compliance were: standard precautions training (odds ratio (OR) 2.17, 95% Akt inhibitor confidence interval (CI) 1.85-2.55) and knowledge (OR 1.94, 95% CI 1.01-3.41), followed by hospital grade (OR 1.61, 95% CI 1.79-1.86), presence of sharps disposal box in the department (OR 1.43, 95% CI 1.10-3.41), general self-efficacy (OR 1.29, 95% CI 1.04-1.59), exposure experience (OR 0.69, 95% CI 0.56-0.85), and department in which the nurse worked (OR 1.24, 95% CI 1.05-1.46).

Conclusions: The relevant authorities and hospital infection control department should pay more attention to nurse compliance with standard precautions.

Standard precautions training should be strengthened and sufficient GSK2879552 supplier practical personal protection equipment provided in order to reduce hospital infections and protect the health of patients and medical staff. (C) 2010 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.”
“Purpose: To analyze the direct relationship between complex internal magnetic resonance (MR) signal intensity (SI) patterns observed in colorectal liver metastases and their microscopic tissue characteristics.

Materials and Methods: The institutional ethics board approved this study. In seven consecutive patients undergoing hepatic resection for liver metastases (primary colorectal in six, breast mistaken for colorectal in one), the resected fresh ex vivo liver specimen was examined with T1-weighted (repetition time msec/echo time msec, 9/4.4-4.8) and T2-weighted (2500/90) MR imaging by using a voxel size of 0.47 x 0.

815). In the probiotic group, the total eosinophil count was significantly lower at the end of the intervention compared to the baseline measurements (p = 0.023). Logarithmic AZD7762 ic50 IFN-gamma and IL-4 were significantly decreased by the end of the intervention compared to baseline measurements in the probiotic group (p < 0.001 and 0.049).

Conclusions Our results suggest that supplementation with probiotic L. plantarum CJLP133 is beneficial

in the treatment of pediatric AD.”
“Purpose of review

Patients with locally ‘advanced’ or muscle invasive bladder cancer have higher mortality rates than patients with nonmuscle invasive (‘superficial’) bladder cancer. Biomarkers can stratify clinical outcomes and thus promise to more 3-MA mouse accurately prognosticate and thus help assign patients

to the appropriate treatments. The aim of this review is to summarize biomarker developments in the past year and to discuss their implications in prognosis and treatment selection in locally advanced bladder cancer.

Recent findings

Prognostic biomarkers for bladder cancer are identified at the DNA, RNA and/or protein levels. Some are new markers, whereas others are established markers with new roles in bladder cancer. Markers can report on the risk of disease recurrence or metastasis, or treatment responsiveness and thus are useful in determining ‘who to treat’ and ‘what to treat with’.

Summary

The list of biomarkers for prognosis and treatment selection for advanced bladder cancer is growing. For most, their clinical relevance is unclear due to their lack of validation in external datasets. MicroRNAs and new techniques including next-generation sequencing offer additional opportunities for biomarker discovery, validation, and clinical applications.”
“Hypothesis: Serotonergic neurons are activated during salicylate-induced tinnitus and modulate the cochlea during tinnitus.

Background: During salicylate-induced tinnitus in the gerbil, neurons in the dorsal raphe nucleus were activated. Because approximately half of the neurons in this region are serotonergic, Danusertib this indicates that serotonin (5-HT) might play a role in the mechanisms of central

tinnitus. The goal of this study was to determine if serotonergic neurons are activated during salicylate-induced tinnitus. Furthermore, to determine if the same neurons might modulate the cochlea during tinnitus, neuroanatomic tract-tracing with 5-HT immunohistochemistry was used to determine if serotonergic neurons project to the gerbil cochlea.

Methods: A randomized, prospective study was performed. Six gerbils were injected with salicylate (saline for controls). Four hours later, the gerbils were euthanized and perfused, and their brains were collected for immunohistochemical labeling of 5-HT and c-fos. For the tract-tracing, FluoroGold was injected into the cochleae of 3 gerbils. The gerbils were euthanized and perfused 4 to 11 days later, and the brains immunohistochemically were processed for 5-HT.

Recent studies have conferred a new acronym to these HBV pol/S gene overlap mutants: ADAPVEMs, for antiviral drug-associated potential vaccine-escape mutants. The present study aimed to assess the prevalence

and pattern of ADAPVEMs in Turkish patients with chronic hepatitis B (CHB).

Methods: The investigation was conducted between March 2007 and July 2010 and involved a total of 442 patients. These patients were in the following phases of HBV infection: immune tolerant (n = 50), immune reactive (n = 37), inactive carrier (n = 90), HBeAg-negative CHB (n = 217), and HBsAg-negative (n = 12), or were hemodialysis patients (n = 36). One hundred eighty-six GDC-0994 molecular weight patients were receiving nucleos(t)ide analogue (NUC) therapy and 256 patients had treatment-naive CHB.

Results: Seven types of ADAPVEM were detected in the total CHB patients: rtM204 V/sI195 M, rtM204I/sW196S, rtM204I/sW196L, rtV173L/sE164D, rtA181T/sW172*, rtA181T/sW172L, and rtA181 V/sL173F. The ADAPVEMs were associated with lamivudine, telbivudine, and adefovir. The prevalence of ADAPVEMs in all CHB patients was found to be 10% (46/442). The difference

in the prevalence of ADAPVEMs across the different CHB clinical phases was not significant (Pearson Chi-square, p = 0.112). The prevalence of ADAPVEMs was 24% (44/186) in those undergoing NUC therapy and 0.7% (2/256) in the treatment-naive group; check details this difference was significant (Pearson Chi-square, p = 0.00).

Conclusions: We determined the prevalence

and pattern of ADAPVEMs in Turkish patients in the different phases of CHB. Preferred drugs in Turkey, such as lamivudine, have the potential to cause the emergence of ADAPVEMs, with the possibility that these will spread to both individuals immunized with the hepatitis B vaccine and nonimmunized individuals. ADAPVEMs should be monitored in infected and treated patients and their public health risks assessed. (C) 2011 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.”
“The perineal body is an important structure which is often injured during labor. It is believed to play a role in pelvic organ support. Vaginal Sapitinib cell line delivery is likely to increase the mobility of perineal body and anorectal junction. The aim of this study was to determine changes in the mobility of perineal body and anorectal junction before and after delivery using pelvic floor ultrasound.

Two hundred nulliparous women were enrolled and underwent pelvic floor ultrasound at 36-38 weeks gestation and 3-6 months postpartum. Levator hiatal dimensions and mobility of the perineal body and anorectal junction were measured in volume ultrasound datasets using postprocessing software, blinded against all clinical data, before and after childbirth.

Ultrasound measures of mobility of perineal body and anorectal junction were shown to be reproducible (ICC 0.74 and 0.76).

For these reasons some chemical strategies are focused in back

to the nature approaches or, in other words, in improving the properties of natural polymers by chemical modifications.

We report that by using a simple chemical modification technique we can obtain new biomaterials, specifically suitable for biomedical applications. Concretely, we describe the chemical modification of gelatin and the suitable characteristics of the modified protein to develop new nanomedicines. This protein was selected because of its enormous potential in biomedicine, which is currently limited due to the difficulty of its use without toxic chemical crosslinkers. The modification of the protein was based on the transformation of the carboxylic group into amido groups selleck products after their reaction with polyamines, leading selleck chemical to a positively charged biopolymer. To cationize the gelatin two polyamines where used: ethylenediamine and spermine, the latter being one of the endogenous polyamines which

has a very positive influence over cells. This modification was monitored by physico-chemical techniques such as NMR, spectrophotometry and potentiometry.

With the most promising modified gelatins we were able to develop nanoparticles using the ionotropic gelation technique. In order to determine the ability of these new nanosystems to associate bioactive molecules we selected a model plasmid DNA. The developed nanosystems were characterized corroborating their ability to associate the genetic material. In conclusion, we were

able to obtain a semi-synthetic biomaterial with tunable physico-chemical properties, which can be used to develop new nanosystems with the ability to associate genetic material. We therefore propose that the gelatin, with its chemical modification, provide a unique biomaterial for the development of new nanomedicines.”
“The temperature-dependent transformation of pi-pi-stabilized stacked malvidin-catechin structures towards polymeric-type structures was studied by photoluminescence (PL) and anisotropy decay studies. The results show an immediate Cytoskeletal Signaling inhibitor decrease of the anisotropy decay after elevating the temperature from 293 K to 303 K or 313 K, while only a very slow increase in the anisotropy decay could be observed when the samples were kept at these elevated temperatures for a longer time such as a few days. The slow polymerization of the malvidin-polyphenol system could be identified as the main process responsible for the change in optical properties by the comparison of the time-dependence of the anisotropy decay with the change observed in the PL intensity. Measurements were also conducted in red wines, where we observed similar results. This suggests a possibly broader relevance of our results for all systems, where anthocyanins and polyphenols are present in considerable concentrations. (C) 2010 Elsevier Ltd. All rights reserved.

(C) 2011 American Institute of Physics. [doi:10.1063/1.3594716]“
“Objectives: To provide an overview on the efficacy, tolerability, safety and health-related quality of life (HRQoL) of drugs with a mixed action used in the treatment of overactive bladder (OAB). Evidence Acquisition: MEDLINE database and abstract books of the major conferences were searched for relevant publications from 1966 to 2011 and using the key words ‘overactive bladder’, ‘detrusor overactivity’, ‘oxybutynin’, ‘propiverine’, and ‘flavoxate’. Two independent reviewers considered publications for inclusion and extracted relevant data, without performing

a meta-analysis. Evidence Synthesis: Old and conflicting data do not support the use of flavoxate, while both propiverine and oxybutynin were found to be more effective than placebo in the treatment of www.selleckchem.com/products/AZD6244.html OAB. Propiverine was at least as effective as oxybutynin but with a better tolerability profile even in the pediatric

setting. Overall, no serious adverse event for any product was statistically significant compared to placebo. Improvements were seen in HRQoL with treatment by the oxybutynin transder-mal delivery system and propiverine extended release. Conclusions: Smad inhibitor While there is no evidence to suggest the use of flavoxate in the treatment of OAB, both oxybutynin and propiverine appear efficacious and safe. Propiverine shows a better tolerability profile than oxybutynin. Both drugs improve HRQoL of patients affected by OAB. Profiles of each drug and dosage differ and should be considered in making treatment choices. Copyright (C) 2012 S. Karger AG, Basel”
“A detailed optical characterization of a freestanding wurtzite semi-insulating Fe-doped GaN (SI-GaN:Fe) grown by hydride-vapor-phase-epitaxy was carried out by photoluminescence (PL) and contactless electroreflectance Nutlin-3a mouse (CER) at 10 and 300 K, respectively, and photoreflectance at 300 K. Low-temperature PL spectrum of the Ga-face consisted of the Fe3+ impurity characteristic series of IR peaks with a sharp zero-phonon line (ZPL) at 1.299 eV, yellow and blue broad emission bands, and near-band-edge

(NBE) emission in the ultraviolet region. The narrow linewidth of 135 mu eV of the ZPL and the measured energy difference of similar to 70 meV between the ZPL and E-2 (high) phonon replica, which is sensitive to the lattice strain, shows good crystal quality and a strain-free incorporation of iron. The obtained transition energies of A, B, and C excitonic features in the CER spectra and the n = 2 excited states (2s) of the A and B excitons enable the estimation of the exciton binding energies. In addition to the free excitonic recombination, the PL spectrum of the Ga-face exhibited clear donor and acceptor related features in the NBE region, while the N-face exhibited a broad emission band related to the free-to-bound recombination only.

Results. Access to the superior joint cavity was achieved without correcting the puncture point in 97% of patients who underwent IGPT and 82% of patients in the CPT group. Significant differences were seen in 1-week maximal mouth opening and pain threshold between IGPT and CPT groups (P < .05 each) and resetting of the puncture point was significantly

Bromosporine nmr less frequent using IGPT compared with CPT (P < .05).

Conclusions. IGPT is effective for pain mitigation and improves mouth opening during the early postoperative period after pumping manipulation treatment. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;111:641-648)”
“Large mammal faunas in tropical forest landscapes www.selleckchem.com/products/JNJ-26481585.html are widely affected by habitat fragmentation and hunting, yet the environmental determinants of their patterns of abundance remain poorly understood at large spatial scales. We analysed population abundance and biomass of 31 species of medium to large-bodied mammal species at 38 Atlantic forest sites (including three islands, 26 forest fragments and six continuous forest sites) as related to forest type, level of hunting

pressure and forest fragment size using ANCOVAs. We also derived a novel measure of mammal conservation importance for each site based on a “”Mammalian Conservation Priority index”" (MP(i)) which incorporates information on species richness, population abundance, body size distribution, conservation

status, and forest patch area. Mammal abundance was affected by hunting pressure, whereas mammalian biomass of which was largely driven by ungulates, was significantly influenced by both forest type and hunting pressure. The MP(i) index, when separated into its two main components (i.e. Selleck AG-881 site forest area and species-based conservation index C(i)), ordered sites along a gradient of management priorities that balances species-focused and habitat-focused conservation actions. Areas with the highest conservation priority were located in semi-deciduous forest fragments, followed by lowland forests. Many of these fragments, which are often embedded within large private landholdings including biofuel and citrus or coffee crops, cattle ranches and pulpwood plantations, could be used not only to comply with environmental legislation, but also enhance the prospects for biodiversity conservation, and reduce edge effects and hunting. (C) 2009 Elsevier Ltd. All rights reserved.”
“Selected reaction monitoring mass spectrometry has been combined with the use of an isotopically labelled synthetic protein, made up of proteotypic tryptic peptides selected from parasite proteins of interest. This allows, for the first time, absolute quantification of proteins from Plasmodium falciparum.