Therefore, to enable averaging of plots of voltage http://www.selleckchem.com/hydroxysteroid-dehydrogenase-hsd.html or AP frequency against current-density, the plot for each cell was interpolated using equally-spaced points (0.5 or 0.1 pA/pF interval) and interpolated values were averaged. The Shapiro–Wilk test was used to determine if data were normally distributed, before choosing a statistical test to compare differences using Origin or GraphPad Prism (La Jolla, CA) or SPSS (Chicago, IL). Differences were considered significant at p < 0.05. Data are summarized as mean ± standard error of the mean (SEM) or median and interquartile values (in parentheses),
with n denoting number of cells. Symbols and error bars in figures represent mean ± SEM. This work was funded by the Wellcome Trust. MMU was in receipt of a Wellcome Trust Research Leave Award. We thank Derek Garden and Jon Brown for comments on early versions of this manuscript, and Jon Brown for help with measurement of instantaneous frequency. Some of the genotyping was carried out by Rachel Davies. “
“The authors regret that the fifth author’s name, “Hai Ying Li” was
incorrectly displayed. It should have appeared as “Haeyeong Lee”. “
“In Table 1, the author has misreported the mean scores of 3 variables in the original article. This does not change the results or the discussion. However, the authors would like to apologise for any inconvenience caused. Updated Table 1 is as follows: “
“Please note that Figs. 1 and 2 should appear as shown below: “
“Important inhibitory mechanisms in the control of water, and particularly NaCl, intake are located in the lateral parabrachial nucleus (LPBN), a pontine structure that lies dorsolateral drug discovery to the superior cerebellar peduncle (Edwards and Johnson, 1991, Menani and Johnson, 1995, Colombari et al., 1996, Menani et al., 1996, Menani et al., 1998a, Menani et al., 1998b and Menani et al., 2000). Early studies ADAMTS5 showed that bilateral injections of methysergide, a serotonergic receptor antagonist, into the LPBN increased water and 1.8% NaCl intake induced by angiotensin II (ANG II) administered either intracerebroventricularly (i.c.v)
or into the subfornical organ (SFO) (Colombari et al., 1996 and Menani et al., 1996). Methysergide injected bilaterally into the LPBN also increased NaCl intake induced by subcutaneous (s.c.) injection of the diuretic, furosemide (FURO), in combination with a low dose of the angiotensin converting enzyme inhibitor, captopril, whereas 2,5-dimetoxy-4-iodoamphetamine hydrobromide (DOI) (a serotonergic 5-HT2A/2C receptor agonist) into the LPBN reduced NaCl intake induced by FURO + captopril (Menani et al., 1996). In addition to serotonin, cholecystokinin (CCK) injected into LPBN inhibited NaCl and water intake (Menani and Johnson, 1998). These studies suggested that signals that inhibit sodium intake are integrated in the LPBN, and involve the release of serotonin and CCK in this area.