FEMS Microbiol Lett 1999, 171:1–9 PubMedCrossRef 13 Huddleston A

FEMS Microbiol Lett 1999, 171:1–9.PubMedCrossRef 13. Huddleston AS, Cresswell N, Neves MCP, Beringer JE, Baumberg S, Thomas DI, Wellington EMH: Molecular detection of streptomycin-producing streptomycetes in Brazilian soils. Appl Environ Microbiol 1997, 63:1288–1297.PubMed 14. Gupte M, Kulkarni P, Ganguli BN: Antifungal antibiotics. Appl Microbiol Biotechnol 2002, 58:46–57.PubMedCrossRef 15. Poole EJ, Bending GD, Whipps JM, Read DJ: Bacteria associated with Pinus sylvestris-Lactarius rufus ectomycorrhizas and their effects on mycorrhiza formation in vitro. New Combretastatin A4 molecular weight Phytol 2001, 151:743–751.CrossRef

16. Ames RN: Mycorrhiza development in onion in repsonse to inoculation with chitin-decomposing actinomycetes. selleck screening library New Phytol 1989, 112:423–427.CrossRef 17. Abdel-Fattah GM, Mohamedin AH: Interactions between a vesicular-arbuscular mycorrhizal fungus ( Glomus intraradices

) and Streptomyces coelicolor and their effects on sorghum plants grown in soil amended with chitin of brawn scales. Biol Fertil Soils 2000, 32:401–409.CrossRef 18. Maier A, Riedlinger J, Fiedler H-P, Hampp R: Actinomycetales bacteria from a spruce stand: characterization and effects on growth of root symbiotic, and plant parasitic soil fungi in dual culture. Mycol Prog 2004, 3:129–136.CrossRef 19. Schrey SD, Salo V, Raudaskoski M, Hampp R, Nehls U, Tarkka MT: Interaction with mycorrhiza helper bacterium Streptomyces sp AcH 505 modifies buy MK5108 organisation of actin cytoskeleton in the ectomycorrhizal fungus Amanita muscaria (fly agaric). Curr Genet 2007, 52:77–85.PubMedCrossRef 20. Schrey SD, Schellhammer M, Ecke M,

Hampp R, Tarkka MT: Mycorrhiza helper bacterium Streptomyces AcH 505 induces differential gene expression in the ectomycorrhizal fungus Amanita muscaria . New Phytol 2005, 168:205–216.PubMedCrossRef 21. Lehr NA, Schrey SD, Bauer R, Hampp R, Tarkka only MT: Suppression of plant defence response by a mycorrhiza helper bacterium. New Phytol 2007, 174:892–903.PubMedCrossRef 22. Deveau A, Palin B, Delaruelle C, Peter M, Kohler A, Pierrat JC, Sarniguet A, Garbaye J, Martin F, Frey-Klett P: The mycorrhiza helper Pseudomonas fluorescens BBc6R8 has a specific priming effect on the growth, morphology and gene expression of the ectomycorrhizal fungus Laccaria bicolor S238N. New Phytol 2007, 175:743–755.PubMedCrossRef 23. Tarkka MT, Herrmann S, Wubet T, Feldhahn L, Recht S, Kurth F, Mailänder S, Bönn M, Neef M, Angay O, et al.: OakContigDF159.1, a reference library for studying differential gene expression in Quercus robur during controlled biotic interactions: use for quantitative transcriptomic profiling of oak roots in ectomycorrhizal symbiosis. New Phytol 2013, 199:529–540.PubMedCrossRef 24. Richard F, Millot S, Gardes M, Selosse MA: Diversity and specificity of ectomycorrhizal fungi retrieved from an old-growth Mediterranean forest dominated by Quercus ilex. New Phytol 2005, 166:1011–1023.PubMedCrossRef 25.

The authors found a significant increase in the expression of a m

The authors found a significant increase in the expression of a microRNA cluster (Smad inhibitor hsa-miR-371-373) in the cisplatin resistant situation, which triggeres p53 silencing [21]. Thus, a future perspective in the field of cisplatin resistance research might be to investigate microRNAs. Thiol-containing proteins and Cisplatin resistance Among various mechanisms of platinum resistance, thiol-containing proteins are of special interest. SIS3 Platinum-based complexes are the only heavy metal containing EMA- and FDA-approved cytostatics at present. This leads to a

very uncommon possible mechanism of resistance: direct interaction of Cisplatin with thiol-groups forming a virtually insoluble sulphide. Since, this mechanism of action in resistance formation is exclusive to platinum-based compounds, it is referred to in this article with a special chapter. Glutathione

or metallothioneins are cysteine-rich peptides, capable of detoxicating the highly reactive aquo-complexes. Cisplatin resistance in ovarian cancer was reported directly proportional to increased intracellular glutathione [22]. However, increased glutathione levels are reversible but resistance is not. Upstream of gluthatione are further thiol-containing proteins called thioredoxins. Mammalian thioredoxins are a family of 10-12 kDa proteins characterized by a common active site: Trp-Cys-Gly-Pro-Cys. Proteases inhibitor Thioredoxin-1 (TRX) is a 12 kDA ubiquitous protein of 104 amino acids with disulfide reducing activity [23]. TRX is frequently found in the cytoplasm, but was also identified in the nucleus of benign endometrial stromal cells, tumour derived cell lines, and primary tumours [24]. Its active site comprises two cystein residues in the consensus sequence serving as a general disulfide oxido-reductase. These two cystein residues (Cys-32, Cys-35) can reversably be oxidized to form a disulfide bond and Chlormezanone be reduced by TRX reductase and NADPH

[25]. The TRX system comprises TRX reductase, NADPH, and TRX itself. It is conserved throughout evolution from procaryotes to higher eucaryotes. The TRX system and the glutathione system constitute important thiol reducing systems [26]. TRX originally was identified as a hydrogen donor of ribonucleotide reductase in Escherichia coli [27]. Targeted disruption of the TRX gene in Saccharomyces cervisiae prolonged the cell cycle [28]. The TRX homologue gene of Drosophila melanogaster was identified as pivotal for female meiosis and early embryonic development [29]. The reducing nuclear environment, caused by thioredoxin, is preferable for the DNA binding activity of various transcription factors such as AP-1 [30], NF-κB [31], and the estrogen receptor [32]. AP-1 activation by TRX also occurs through an indirect mechanism: TRX reduces Ref-1, which in turn reduces cysteine residues within the fos and jun subunits of AP-1, thereby promoting DNA binding [30].

Participants griped the handle in their right hand; the adapter l

Participants griped the handle in their right hand; the adapter length was adjusted so their right arm was fully extended (0°) (i.e. minimal flexion in the elbow). Participants’ movement was restricted by securing Velcro straps across the upper legs and hips with the left arm placed across the chest. The point of rotation of the dynamometer arm was aligned with the right Acromiale [14]. Participants were tested on their right arm only, but very little difference in strength exists between Staurosporine order dominant and non- dominant arms [12]. Range of motion was between 0° and 180°.

The test protocol consisted of 2 sets of 5 maximal dynamic contractions of the shoulder extensors and flexors at 60 and 180°·s-1, each separated by 30 s rest. Food Diary Participants were instructed to consume a light meal (cereal and toast) at least 3 hours prior to treadmill selleck kinase inhibitor walking sessions (PLA: 266 ± 157 Kcal (carbohydrate: 51 ± 37; fat 3 ± 3; protein: 11 ± 6), CHO: 259 ± 154 Kcal (carbohydrate: 49 ± 36; fat 3 ± 3; protein: 11 ±

6), PRO (277 ± 147 Kcal (carbohydrate: 55 ± 34; fat 3 ± 3; protein: 10 ± 6). There were no differences in macronutrient intake prior to treadmill walking between selleckchem conditions (P > 0.05). Participants recorded any food or beverages (with estimated mass or portion size) consumed on the day of and for 72 hours after treadmill walking. Food diaries were analysed using Microdiet Plus for Windows V1.2 (Downlee Systems Ltd, Derbyshire, UK). There were no differences between conditions before or after load carriage in dietary intake of energy (Table 1). Table 1 Dietary intake

of energy, carbohydrate, fat and protein Variable Condition 24 h 48 h 72 h Energy (Kcal) PLA 1494 ± 740 1484 ± 659 1600 ± 549   CHO 1547 ± 702 1468 ± 680 1532 ± 628   PRO 1611 ± 658 1481 ± 626 1613 ± 534 Carbohydrate (g) PLA 212 ± 162 217 ± 159 221 ± 108   CHO 224 ± 156 209 ± 162 207 ± 111   PRO 233 ± 150 216 ± 161 226 ± 106 Fat (g) PLA 41 ± 24 41 ± 28 52 ± 28   CHO 45 Chlormezanone ± 28 45 ± 32 50 ± 26   PRO 46 ± 27 43 ± 23 53 ± 23 Protein (g) PLA 82 ± 26 73 ± 27 76 ± 21   CHO 77 ± 22 69 ± 23 75 ± 22   PRO 80 ± 23 69 ± 19 73 ± 21 Measured by food diaries after (24, 48 and 72 h) 120 minutes of treadmill walking at 6.5 km·h-1 (n = 10) on a level gradient (0%) carrying a 25 kg backpack. Either a placebo beverage (PLA), carbohydrate (6.4%) beverage (CHO) or protein (7%) beverage (PRO) was consumed at 0 and 60 minutes (250 ml) during treadmill walking or twice daily (500 ml, morning and evening) for the 3 days after load carriage (n = 10). Data are presented excluding the consumption of the supplement beverages. Statistical Analysis Statistical analysis was undertaken using SPSS for Windows V15 (SPSS, Chicago, Illinois). Normal distribution of the data was verified using a Kolmogorov-Smirnov test. Differences between groups and over time were assessed using 2 way repeated measures ANOVA. If sphericity was violated, the Greenhouse-Geisser correction was used.

We have attempted a careful manual evaluation in Table 4 The rea

We have attempted a careful manual evaluation in Table 4. The reason for interaction promiscuity and thus false positives remains unclear. Several hypotheses have been proposed to explain such cases. For example, a protein may have hydrophobic patches that interact unspecifically. Some authors have suggested that simply an increase in abundance might cause a promiscuous

gain of interactions [18] but such theories remain to be tested rigorously. The Y2H assay appears Tozasertib manufacturer to be sensitive enough to detect weak interactions that are not detectable in NMR experiments, e.g. the interaction between U monomers [19]. The high sensitivity may also explain a significant number of false positives which may have been detected in our screen but which do not have any physiological significance. Future quantitative measurements are thus CDK inhibitor required to clarify the relationship between affinity and physiological

relevance. Head assembly and structure The structure of the lambda protein shell is known in great detail [20]. However, its assembly is much less well understood as are the locations and functions of the “”minor”" proteins that are present in only a few molecules/virion (Figure learn more 5). The portal protein B is believed to be the nucleator or initiator of head assembly, which first assembles with the C protease and with the scaffolding protein Nu3 into an ill-defined initiator structure. B, C, and Nu3 are known to form a complex in which several interactions have been previously reported ADP ribosylation factor (C’-B, C-Nu3, Nu3-Nu3, and Nu3-B, Table 2). We could not detect B in any interaction although we did find Nu3-C, Nu3-Nu3 and Nu3 interactions with E and Z. This is noteworthy because Nu3-E and Nu3-Z are new interactions. It is known that E (the major capsid protein) assembles onto or around the initiator structure to form the procapsid [12], and it is conveivable that B joins such an assembly. If Nu3 and C proteins are both required for B

to join, we would have missed this interaction, given that we tested only pairs of proteins. Nu3 also appears to form dimers by the Y2H analysis, and this has been confirmed independently (C. Catalano, pers. comm.). Figure 5 Head assembly. Head assembly has been subdivided in five steps although most steps are not very well understood in mechanistic terms. The tail is assembled independently. The C protease, the scaffolding protein Nu3, and the portal protein (B) form an ill-defined initiator structure. Protein E joins this complex but the chaperonins GroES and GroEL are required for that step. Within the prohead C and E are processed to form covalently joined X1 and X2 proteins although this is controversial (see text). Proteins Nu1, A, and FI are required for DNA packaging. Protein D joins and stabilizes the capsid as a structural protein. FII and W are connecting the head to the tail that joins once the head is completed.

The extracted ΦB values of these samples are presented in the Fig

The extracted ΦB values of these samples are presented in the Figure 4. The highest ΦB value attained by the NF-��B inhibitor sample annealed in O2 ambient (3.72 eV) was higher than that of metal-organic decomposed CeO2 (1.13 eV) spin-coated on n-type GaN substrate [20]. No ΦB value has been extracted for the sample annealed in N2 ambient due to the low E B and high J of this sample, wherein the gate oxide breaks down prior to the FN tunneling mechanism. Figure 7 Experimental data fitted well with

FN tunneling model. Experimental data (symbol) of samples annealed in O2, Ar (HJQ and KYC, unpublished work), and FG ambient fitted well with FN tunneling model (line). Table 1 compares the computed ΔE c values from the XPS characterization with the ΦB value extracted from the FN tunneling model. From this table, it is distinguished that the E B of the sample annealed in O2 ambient is dominated by the breakdown of IL as check details the obtained

value of ΦB from the FN tunneling model is comparable with the value of ΔE c(IL/GaN) computed from the XPS measurement. For samples annealed in Ar and FG ambient, the buy A-1155463 acquisition of ΦB value that is comparable to the ΔE c(Y2O3/GaN) indicates that the E B of these samples is actually dominated by the breakdown of bulk Y2O3. Since the leakage current of the sample annealed in N2 ambient is not governed by FN tunneling mechanism, a conclusion in determining whether the

E B of this sample is dominated by the breakdown of IL, Y2O3, or a combination of both cannot be deduced. Based on the obtained values of ΔE c(Y2O3/GaN), ΔE c(IL/GaN), and ΔE c(Y2O3/IL), the E B of this sample is unlikely to be dominated by IL due to the acquisition of a negative ΔE c(IL/GaN) value for this sample. Thus, the E B of this sample is most plausible to be dominated by either Y2O3 or a combination of Y2O3 and IL. However, the attainment of ΔE c(Y2O3/IL) value which is larger than that of ΔE c(Y2O3/GaN) value obtained for the samples annealed in Ar and FG ambient eliminates the latter possibility. The reason behind Sirolimus it is if the E B of the sample annealed in N2 ambient is dominated by the combination of Y2O3 and IL, this sample should be able to sustain a higher E B and a lower J than the samples annealed in Ar and FG ambient. Therefore, the E B of the sample annealed in N2 ambient is most likely dominated by the breakdown of bulk Y2O3. Table 1 Comparison of the obtained Δ E c and Φ B values   XPS: conduction band offset     J-E   Y 2 O 3 /GaN IL/GaN Y 2 O 3 /IL Barrier height O2 3.00 3.77 0.77 3.72 Ar 1.55 1.40 0.15 1.58 FG 0.99 0.68 0.31 0.92 N2 0.70 −2.03 2.73 a aNot influenced by FN tunneling. Therefore, barrier height is not extracted from the FN tunneling model.

3 0 143   CHN-D Nanning, Guangxi province 14 14 3 0 0 276   CHN-E

3 0.143   CHN-D Nanning, Guangxi province 14 14 3.0 0.276   CHN-E Fuzhou, Fujian Province 7 5 2.1 0.320   CHN-F Tangshan, Yunnan Province 3 2 1.3 0.143   CHN-G Gangzhoou, Jiangxi Province 1 1 1.0 0.000

  CHN-H Guangzhou, Guangdong Province 1 1 1.0 0.000   CHN-I Hunan Province 1 1 1.0 0.000   China-overall 36 31 5.7 0.342 CAMBODIA CAM-A Pursat Province 7 6 2.4 0.341   CAM-B Battambang Province 4 4 1.9 0.304   Cambodia-overall 11 10 3.1 0.423 VIETNAM VIET Hung Yen Province, Hoa Binh Province, Hanoi 3 3 1.9 0.317 THAILAND THAI Protein Tyrosine Kinase inhibitor Unknown 1 1 1.0 0.000 TAIWAN TIW Unknown 1 1 1.0 0.000 JAPAN JPN Unknown 1 1 1.0 0.000 INDIA IND-A Anantapur District, Andhra Pradesh 7 7 2.4 0.297   IND-B Chittoor District, Wortmannin manufacturer Andhra Pradesh 6 6 2.0 0.254 MNK inhibitor   IND-C Kadapa District, Andhra Pradesh 4 4 1.9 0.250   IND-D Mahaboobnagar District, Andhra Pradesh 3 3 1.4 0.159   IND-E Nalgonda District, Andhra Pradesh 4 4 1.7 0.196   IND-F Prakasam District, Andhra Pradesh 4 3 2.4 0.540   IND-G Tirupati District, Andhra Pradesh 5 5 2.0 0.274   IND-H Kurnool District, Andhra Pradesh 1 1 1.0 0.000   IND-I Ludhiana District, Punjab 1 1 1.0 0.000   India-overall 35 34 5.4 0.360 Allele per locus: average number of alleles per locus Clone corrected data (removed repeated genotypes within a population) Genotype and genetic diversity A total of 117 genotypes (haplotypes) were identified

(Additional file 1). Haplotypes identified within the sample population were restricted to the boundaries of their country of origin. The genetic diversity observed in different countries and locations are summarized in Table 2. Isolates from China possessed the largest number of alleles (5.7 alleles per locus), followed by India (5.4 alleles per locus). Overall

haploid genetic diversities were the highest in Asian countries, followed by Brazil. The haploid genetic diversity of the Florida (USA) isolates was lowest among all the geographic groupings (Table 2). Genetic structure A UPGMA clustering analysis identified three major groups of ‘Ca. L. asiaticus’ (Figure 1). Isolates from India BCKDHB were clustered in a distinct group (group 3). Most of the isolates from China and other Asian countries, and Brazil were generally grouped in group 1. While some isolates from Florida occurred in group 1, most isolates from Florida were clustered in group 2 (Figure 1). Figure 1 UPGMA dendrogram showing the genetic relationships of ‘ Candidatus Liberibacter asiaticus’ isolates from different locations within an individual country as well as from different countries (from Asia and Americas). Clone-corrected data were used for constructing the dendrogram based on DA distance [22]. Only bootstrap values > 25% are shown. The STRUCTURE analysis based on Bayesian modeling further assessed the genetic structure of ‘Ca. L. asiaticus’. This approach utilizes statistical methods to determine the relationships among the isolates without prior population information.

01 kcal Å−1 The following molecular descriptors taken from Hyper

01 kcal Å−1. The following molecular descriptors taken from HyperChem software were

considered among quantum and chemical indices: total energy (TE), binding energy (BE), isolated atomic energy (IAE), electron energy (EE), core–core energy (CCE), heat flow (HF), energy of the highest occupied molecular orbitals (E_HOMO), energy of the lowest unoccupied molecular orbitals (E_LUMO), and difference between HOMO and LUMO energies www.selleckchem.com/products/tpca-1.html referred to as EG = energy gap; ionization energy (potential) (IE) and electron affinity (EA) were calculated as a difference between the HF of positive molecular ion and electrically neutral molecule, and electronegativity (EN) calculated as average arithmetic potential of ionization and EA. In addition, other parameters were used as the value of electron density of atom orbitals from the lowest to the highest (ED_MIN and ED_MAX, respectively), the highest positive electron charge on the atoms (MAX_POS),

and the highest negative electron charge on the atoms (MAX_NEG), the difference between the highest positive and negative charge (DELTA_Q), distribution of dipolar moment along x, y, and z axes (X_DM, Y_DM, and Z_DM, respectively), total dipolar moment (TDM), mean polarizability of molecules (in atom units) MP (Mean Polarizability), energy equal to the length of the wave with the greatest long-wave transfer of electrons, for which the Selleck C188-9 value of oscillator force was different from zero (EL)—the value of

wave figures were converted to eV—and the value of the most intensive electron transfer (EMAX—the maximum value of oscillator force calculated with the use of AM1 method—as well as oscillator maximum force used for the transfer (OS_EMAX). Moreover, additional parameters were calculated with the use of QSAR Properties Module of HyperChem. They include the following descriptors: surface area of the molecule available for solvent (SA), molecule volume (V), hydration energy (HE), the calculated distribution coefficient logarithm (logP), refraction (R), and polarizability (P). Carnitine palmitoyltransferase II On the other hand, using Dragon software, over 1,300 molecular descriptors were calculated and considered for QSAR analysis. They include molecular parameters from different group and class of descriptors as constitutional, topological, walk and path counts, connectivity indices, information indices, 2D autocorrelations, edge adjacency indices, topological charge indices, eigenvalue-based indices, geometrical, 3D-MoRSE, WHIM, GETAWAY, functional group counts, atom-centred fragments, charge, molecular properties and other group of descriptors, and describing some properties of compound as geometry, symmetry, topology, learn more electronic, steric or thermodynamic and other properties. The definitions of these descriptors are reviewed by Todeschini (Todeschini et al., 2000).

marginatus, and canids for all

marginatus, and canids for all stages of R. sanguineus. Adult H. lusitanicum and D. marginatus normally feed on large ungulates. Animals present in the tick study areas included, apart from cattle, high densities of rabbits and other wildlife. It is to note that 40 liver samples from rabbits hunted in Gran Canaria analyzed by PCR were all negative (data not shown), although more studies are needed. Whether some of the above mentioned animals may act

as reservoirs for GG VII C. burnetii remains to be studied. Interestingly, in 7 cattle samples from 4 distant regions, only GG III was detected. In the study of Arricau-Bouvery [13] most of the cattle isolates (12/14) analyzed by MLVA also grouped together in a clade that is close but different to the one that click here include GG I isolates, as in this study. In Beare’s study GG III is also philogenetically close to GG I and both clades appear together in Cl-amidine supplier the tree. This GG having never been found in humans in Spain so far lead us to hypothesize that cattle could represent a low risk for Q fever transmission to humans

in our country. One of the added values of the method described here is that it could be applied to any PCR-positive sample carrying at least 10 genome equivalents of the target organism, thus avoiding the need for culturing the organism to obtain data on the global circulation Dasatinib in vitro of C. burnetii. The frequent lack of human isolates from outbreaks, which are needed to apply the yet described methods, hamper a correct outbreak study that are necessary to identify the source of infection. This methodology allows the characterization directly from clinical samples avoiding the culture step of this fastidious bacterium, and proves to be valuable identifying so far 10 different GTs circulating in Spain. This method can be performed in any laboratory with basic equipment. It can easily determine relationships among C. burnetii from different origins by using PCR-positive samples, thus helping in the

identification of the source of an outbreak in a rapid analysis. Conclusions The method described here is rapid, reproducible and sensitive. It can be applied directly to clinical and environmental samples, and is able to identify up to 16 GT. This Carbohydrate will facilitate the acquisition of global data on the circulation of GT of this organism. We have found a high variability of C. burnetii in Spain, with 10 GTs found in different settings, 5 of them in human samples. Interestingly, all the samples from acute cases of FID with liver involvement were produced by adaA negative microorganisms, while the only case of pneumonia available for the study was caused by a adaA positive strain. Moreover, the majority (12 cases) of the 13 chronic cases studied were produced by organisms of GG IV-, except for a case of vascular infection (GG VIII +). Regarding livestock, human cases share GTs with sheep and goats, but the only GT found in cattle has never been found in humans.

Another alternative approach applied to solution-phase highly mul

Another alternative approach applied to solution-phase highly multiplex PCR has been the replacement of target-specific primers with universal ones. However, this process involves multiple steps starting with enzymatic digestion of the template DNA, ligation to adapters, primer extension and finally two subsequent PCR reactions [30, 31]. Such multi-step approaches are time consuming and prone to contamination

[25] and therefore have not been recommended for bacteriological routine diagnostics. The coupling of a pre-processing multiplex PCR to a medium-density microarray format, displaying hundreds of probes for identification and virulence profile typing of several pathogenic species, SB431542 requires an unbiased multi-target amplification corresponding to several dozens of specific capture probes characterizing a certain pathogen. Since the presence GSK2126458 in vitro and concentration of the particular pathogen in a microbiological laboratory is unknown, the multiplex reaction should include as many primer pairs as capture probes are present on the microarray. Moreover, SRT1720 purchase the reaction has to cope with femtograms of pathogen template DNA whose GC-content can

range between 30 and 70% and which is mixed with nanograms of human DNA. We have shown high fidelity amplification of specific DNA targets using pools of species-specific mixes of up to 800 primer pairs, which improves the sensitivity of the microarray detection of pathogens by a factor of 2 to 3-logs. By using S. aureus DNA (strain ATCC 29213) as template for amplification, we demonstrated that LSplex tolerates the increase in primer mix complexity until at least 800 primer pairs, without significant reduction filipin in the profiling fidelity. LSplex products amplified from 10 and even 1 ng of template generated fluorescent signals as strong as those produced by micrograms of genomic DNA. Nevertheless, the comparison between LSplex hybridization profiles and the ones obtained with 2 μg of S. aureus showed that some probes were poorly amplified with the high

complexity primer mixes. These probes produced a strong fluorescent signal when hybridized with genomic DNA but upon the LSplex protocol they were not considered as positive since their fluorescence difference was less then 2 times SD to the mean fluorescence intensity of the whole microarray. This problem of under-amplification of some targets might be circumvented by a specific increase in the concentration of primer pairs amplifying these specific targets [32]. Such a balancing strategy for individual primer pairs could be applied on the whole set of primers, following a broad comparison between hybridization profiles generated by genomic DNA of many reference strains of all species of interest and the LSplex amplified products.

Science and Technology) 2007-2011 This work was partly supported

Science and Technology) 2007-2011. This work was partly supported by a research grant for Higashiosaka City. References 1. Tarhini

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