“
“BACKGROUND: Lung transplantation (LTx) is established as a life-saving treatment in end-stage ��-catenin signaling lung disease. However, long-term survival is hampered by the development of chronic rejection, almost synonymous with bronchiolitis

obliterans syndrome (BOS). The rejection is characterized by deposition of extracellular matrix in small airways. Fibroblasts/myofibroblasts are the main producers of extracellular matrix molecules such as proteoglycans. This study compared fibroblast phenotype and activity in the wound healing process at different points after LTx in patients who later did, or did not, develop BOS.

METHODS: Distally derived fibroblasts from patients 6 and 12 months after LTx and from healthy controls were analyzed for production of the proteoglycans versican, perlecan, biglycan, and decorin, with and without transforming growth factor (TGF)-beta(1). Fibroblast migration and proliferation were also studied.

RESULTS: At 6 and 12 months after LTx, versican production was higher in fibroblasts from LTx patients (p < 0.01 p < 0.01) than from controls. Fibroblasts from patients who later developed BOS were more responsive to TGF-beta(1)-induced synthesis of versican

and biglycan than patients without signs of rejection (p < 0.05). Production of perlecan and decorin was negatively correlated with fibroblast proliferation in fibroblasts at 6 months this website after LTx. In a more detailed case study of 2 patients, one with and one without BOS, the altered proteoglycan profile was associated with impaired lung function.

CONCLUSIONS: LTx changes the phenotype of fibroblasts to a non-proliferative but extracellular

matrix producing cell due to wound healing involving TGE-beta(1). If not controlled, this may lead to development of BOS. J Heart Lung Transplant 2011;30:945-54 (C) 2011 International Society for Heart and Lung Transplantation. All rights reserved.”
“The DLTS signal induced by a back contact barrier is studied both theoretically and through experiments on model circuits. A nonideal back contact is modeled either by a resistor and a capacitor, or by a germanium diode inversely polarized Belinostat in vivo with respect to the junction diode. Depending on the back contact properties, this may result in a positive or negative capacitance transient. For these model circuits the capacitance transient time constants and amplitudes are studied as a function of voltage pulse height and compared with signals originating from emission and slow capture from a defect level. These two origins of DLTS signals present very different properties, which opens possibilities to distinguish between them. (C) 2011 American Institute of Physics. [doi: 10.1063/1.