The recombinant aprotinin had the same characteristics as bovine selleck kinase inhibitor aprotinin in a number of analytical methods, including alpha 2-plasmin inhibition assay, amino acid composition, N-terminal amino acid sequence determination, and mass spectrum analysis.
With further optimization of the purification process and culture conditions for high-yield production by S. cerevisiae, this source of recombinant aprotinin may be a promising approach for the commercial manufacture of aprotinin for pharmaceutical use instead of bovine aprotinin. (C) 2009 Elsevier Inc. All rights reserved.”
“Transplantation of bone marrow stromal cells (BMSCs) is a potential therapy for ischemic stroke, but poor environmental conditions in brain lesions, such as insufficient nutrition and oxygen free radical toxicity, limit the see more efficacy of stem cell therapy. Here, we hypothesized that MCI-186, a free radical scavenger, would have protective effects on transplantation of BMSCs in a rat ischemia model. In vitro, flow cytometry showed the apoptotic rates of BMSCs after simulated ischemia-reperfusion (I/R) injury was significantly decreased when treated with MCI-186 (P < 0.01). In vivo, rat transient middle cerebral artery occlusion (MCAO) model was established.
Two separate MCAO groups were administered with either MCI-186 or phosphate-buffered solution (PBS) immediately after artery occlusion. MCI-186 significantly up-regulated the secretion of brain-derived neurotrophic factor, vascular endothelial growth factor and superoxide dismutase in ischemic brain, while malondialdehyde decreased and neuronal apoptosis was inhibited. Furthermore, another four MCAO groups were administered with either PBS, MCI-186, BMSCs (2 x 106) or a combination of MCI-186 and BMSCs. When compared with BMSCs or MCI-186 monotherapy, combination therapy significantly improved functional restoration, decreased infarct
volume, and increased the number of engrafted-BMSCs and neurons in ischemic brain. The number of engrafted-BMSCs and neurons was Arachidonate 15-lipoxygenase significantly correlated with functional outcomes. This study suggests that MCI-186 may improve the environment of the injured brain, enhance the survival of engrafted-BMSCs and neurotization in ischemic brain and produce protective effects on BMSCs transplantation. Crown Copyright (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.”
“We report here the complete genomic sequence of the Chinese duck flavivirus TA strain. This work is the first to document the complete genomic sequence of this previously unknown duck flavivirus strain. The sequence will help further relevant epidemiological studies and extend our general knowledge of flaviviruses.