The arrow with the solid line represents the cytoplasmic Wolbachia PCR product restricted to the reproductive click here tissues, and the arrow with the dashed line represents

the PCR product found in all tissues tested. A 100 bp DNA ladder is used as size marker Discussion Prevalence of Wolbachia in Glossina species Our study suggests that Wolbachia infections are present in multiple species of the genus Glossina; however, the prevalence of infections in laboratory colonies versus natural populations and the Wolbachia strain harboured in the different species varies. The infection seems to be prevalent to the morsitans (savannah) group, which includes the species G. m. morsitans, G. m. centralis and G. austeni. In addition, uncured www.selleckchem.com/products/azd2014.html laboratory colonies largely show fixation, suggestive of active cytoplasmic

incompatibility (Alam and Aksoy, personal communication). Wolbachia was also detected in the fusca (forest) group, which includes G. brevipalpis. In contrast, Wolbachia infection seems to be largely absent from the palpalis (riverine) group, which includes G. f. fuscipes, G. tachinoides and G. p. palpalis. It should be mentioned, however, that our results depend on the PCR-amplification conditions employed in this study and the presence of low density Wolbachia infections in these species, as has been reported for other insect species [66–68], cannot be excluded. Given that our screen was based on specimens collected during 1994-2010 (see Table 1), new screens should provide information on the dynamics of infection and the expression of cytoplasmic incompatibility. The abovementioned fantofarone data are in accordance with previous reports that detected Wolbachia in G. m. morsitans, G. m. centralis, G. ARS-1620 molecular weight brevipalpis and G. austeni [42, 43].

For the first time our study reports the presence of Wolbachia, albeit at very low prevalence, in G. pallidipes (morsitans group) and in G. p. gambiensis (palpalis group). The infection was only detected in 22 out of 1896 G. pallidipes and in 2 out of 644 G. p. gambiensis individuals; in both species, the infection was present in different populations, as shown in Table 1. Whether the presence of Wolbachia in these two species is a result of horizontal transfer, hybrid introgression or co-divergence in the morsitans and palpalis species complexes, as has recently been shown in other species complexes, has to await investigation [69–71]. The prevalence of Wolbachia was not homogenous among the different natural populations of G. m. morsitans. For example, in the area Gokwe (Zimbabwe), the infection prevalence was almost nine times lower than the average of the other areas. Glossina populations have been shown to exhibit extensive genetic structuring; of which the observed Wolbachia infection dynamics may be a result [72, 73]. Similar observations were made in G.