Several methodologies exist for the construction of phylogenetic

Several methodologies exist for the construction of phylogenetic trees: single gene trees, trees based on concatenated gene sequences, gene content trees, and gene order trees. Phylogenetic trees based on single genes are unlikely to provide an accurate lineage of the serovars because of horizontal gene transfer among ureaplasmas. We find extensive horizontal gene transfer among clinical isolates relative to the 14 ATCC type strains [26]. Another challenge of building intra-species phylogenetic

trees based on a single gene is that the primary nucleotide sequences of the genes conserved among all ureaplasma serovars/strains have such a high percentage of identity that there are not enough informative positions in the multiple sequence alignment to provide

EGFR inhibitor a resolution capability with high confidence. A gene content tree is based on a multiple sequence alignment in which each sequence (line) represents PR-171 in vivo the genome of a strain and each position (column) in the multiple sequence alignment signifies the presence or absence of a gene in the strain. Therefore, such a tree has a binary nature (presence = 1, absence = 0). The pan genome of ureaplasmas generates a relatively short multiple sequence alignment: 1020 positions for 1020 genes in the pan genome. Therefore, a gene content tree of ureaplasma strains does not have the fine resolution capability of a phylogenetic tree based on nucleotide sequences. This can be noted in the low bootstrap values of the deep nodes of the gene content tree based

on the pan genome (Additional file 4: Table S1). We did not attempt to construct a gene order tree, because the majority of the P-type ATPase genomes are in multiple pieces, thus making it hard to judge the gene order in these genomes. Phylogenetic trees of ureaplasmas have been published previously, showing clear separation of the parvum and urealyticum species [27, 28]. The conserved buy OSI-906 domain of the mba genes has been used to generate a phylogenetic tree to resolve the relationship of serovars [5, 29]. We reconstructed the mba conserved domain tree using the first 430 nucleotides of the mba gene of all 19 strains (Figure  3). We also present a phylogenetic tree (Figure  4) based on the information of the nucleotide sequence of 82 housekeeping genes forming four groups: 1) 16 tRNA ligase genes 2) 12 RNA and DNA polymerase genes, 3) 47 ribosomal protein genes, and 4) 7 ureases. The clades of the multigene tree are very similar to the clades of the previously published mba based tree; however, the deep nodes of the two trees show some differences.

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