Key Word(s): 1. lncRNAs; 2. hypoxia; 3. gastric cancer; 4. metastasis; Presenting
Author: DULCIENEM.M. QUEIROZ Additional Authors: GIFONEA ROCHA, ANDREIAMC ROCHA, NATHALIEB.F. ALMEIDA, VIVIANEC.F. SANTOS, KÁDIMAN. TEIXEIRA, FABRÍCIOF. MELO, ALDOA.M LIMA Corresponding Author: DULCIENEM.M. QUEIROZ Affiliations: Universidade Federal de Minas Gerais; Unviersidade Federal de Minas Gerais; Unviersidade Federal do Ceará Objective: In genome-wide association studies, STAT3 (rs744166) minor G allele is associated with protection against inflammatory selleck inhibitor bowel disease. It is well known that STAT3 has protective effect on the intestinal mucosa, whereas in the stomach it is associated with carcinogenesis by participating in a series of tumorigenic processes such as cell proliferation, survival, anti-apoptosis, angiogenesis and immune evasion and inflammation. Thus, we evaluated whether the polymorphism is associated with increased risk of gastric cancer. Methods: We included 878 subjects: 232 H. pylori-positive adult patients with distal gastric carcinoma and 105 with gastritis and 541 voluntary blood donors. The STAT3 polymorphism was analyzed by Taqman SNP genotyping assays in DNA extracted
from leukocytes. Cytokine gastric concentrations were evaluated according to each genotype in the group of patients with gastritis VX 809 by ELISA. Data were analyzed with SPSS version 17. Results: The alleles at individual loci were in Hardy-Weinberg equilibrium in the control group. The frequency of G allele was higher (p = 0.01) in gastric cancer patients (52.6%) than in the control group (43.4%). In the multivariate analysis, the rs744166 minor allele (OR = 1.68, 95%CI = 1.17 – 2.41), increasing
age (OR = 1.20, 95%CI = 1.17 – 1.23) and male gender (OR = 1.75, 95%CI = 1.01 – 3.05) remained independently associated with gastric cancer. The gastric concentrations of IL-11, IL-17A and IL-23 were higher in the carriers of the rs744166 polymorphic allele than in those with the wild genotypes. Conclusion: Concluding, STAT3 rs744166 polymorphism is a risk factor for gastric cancer and modifies the gastric expression of Th-17 cell-related Anidulafungin (LY303366) cytokines, which may be due to the role of STAT3 in controlling Th-17 pathway. Grants: INCT/CNPq/CAPES. Key Word(s): 1. Helicobacter pylori; 2. Gastric cancer; 3. Stat3; 4. Interleukins; Presenting Author: HONG LI Additional Authors: DAIMING FAN, YONGQUAN SHI, JIE DING Corresponding Author: YONGQUAN SHI, JIE DING Affiliations: Xijing Hospital of Digestive Diseases & State Key Laboratory of Cancer Biology Objective: Gastric intestinal metaplasia (IM) is the most common precancerous lesion of gastric cancer. Bile acids have been considered playing a pivotal role in the induction of IM. However, the underlying mechanisms have not been clearly understood. We aimed to explore the role of miRNAs in bile acids induced IM.