http://www.selleckchem.com/products/Sunitinib-Malate-(Sutent).html 3. About 800 genes were found to be differentially regulated in the two sample groups 537 with upregulation and 258 with downregulation in AMC. A high number of differentially expressed genes identified were either novel or did not have any functional annotation. In view of this, a list of genes with known functional annotations was generated using the statistical functions in the Bioinformatics Inhibitors,Modulators,Libraries Tool box in MATLAB R2009a. This list was generated using a less Inhibitors,Modulators,Libraries stringent filtering and contained close to 1400 upregulated genes and 700 downregulated genes in AMC compared to RMC. Functional categorization of AMC and RMC The genes with highest fold change values in AMC and RMC clearly delineate their functions and residing environment.
For example, the AMC express genes that are shown to be involved in nervous system de velopment, immune system development, cell migration during neurodevelopment and the immune response as well in migration of microglia. The finding of expression of some genes that are known to be neuron specific, Inhibitors,Modulators,Libraries is interesting and has been further confirmed by immunohistochemical analysis which revealed the expression of Dcx by the AMC in the CC of 5 day old rat brain. In addition, mRNA expression of Dcx was detected in the BV 2 micro glia by RTPCR. Interestingly, the RMC express genes involved in myelin ation. Mbp like proteins, also known as Golli proteins have previously been shown to be localized in human microglia at 22 weeks postnatally. MBP mRNA was found to be expressed by laser captured RMC and BV 2 microglia.
Other myelin related genes like Plp1 and Lgi4 have also been found with high expression values in the RMC. Plp1 and Lgi4 were found to be expressed by non Inhibitors,Modulators,Libraries myelinating cells such as the Bergmann glia in cerebellum of the developing mouse brain. On sorting the genes based on p values, we found several genes that are specific to AMC such as, genes involved in transcriptional repression, vesicular trafficking, and microtubule depolymerization. RMC express genes involved in immune functions such as RT1 A2, which is the MHC of rat and C1ql3, a protein of the complement system, calcium ion signaling pathway protein, Camk2 and sodium dependent glucose transporter gene Slc5a11, Inhibitors,Modulators,Libraries known to interact with immune related genes. bone marrow derived circulating monocytes.
The availability of gene expression profiles for circulating monocytes prompted us to compare and study the func tional check details similarities of AMC and RMC to that of circulat ing monocytes. Monocytic genes highly expressed by AMC are known to be involved in several disease pathways such as Parkinsons disease, Huntingtons disease and HIV infection, phagocytosis and chemokine signaling pathways. Further, the monocytic genes expressed by RMC are involved in antigen presentation and lysosome related functions.