importance for optimal growth of Toxoplasma Dictyostelium develo

importance for optimal growth of Toxoplasma. Dictyostelium development is ultrasensitive to O2 making it a good model for understanding the mechan ism of O2 sensing by other organisms that conserve the Skp1 thoroughly modification pathway. Development is induced by starvation, which signals the normally solitary phagocytic amoebae to form a multicellular fruiting body, which consists of a cellular stalk that aerially supports thou sands of spores for potential dispersal to other locations. Initially, the amoebae chemotax together to form a multicellular aggregate, which polarizes in response to environmental cues and elongates into a migratory slug consisting of prestalk cells mostly at its anterior end and prespore cells in the remainder.

The slug responds to environmental signals that direct its migration and regulate the slug to fruit switch the process of culmination leading to formation of the fruiting body. Signals include light, low NH3, low moisture, higher temperature, and high O2 which, in the native environment of the soil, draw the subterranean slug to above ground where culmination is most pro ductive. In the laboratory, the process takes place over the course of 24 h after deposition of amoebae on moist agar or filter surfaces wetted with low salt buffers. Whereas amoebae grow and form slugs at an air water interface in the presence of as little as 2. 5% O2, 10% is required for culmination, and slugs immersed in mineral oil require atmospheric hyperoxia to culminate. Overexpression of Skp1 or absence of pathway activity drives the O2 requirement up to 18 21%, whereas decreased Skp1 or overexpression of PhyA drives the O2 requirement down to 5% or less.

These genetic manipulations also revealed effects on timing of slug formation and on sporulation. Together with studies on a Skp1 mutant lacking the modifiable Pro143 residue, and double mutants between Skp1 and pathway enzyme genes, the findings suggested that the Skp1 modification pathway mediates at least some O2 responses. However, O2 con tingent modification AV-951 of the steady state pool of Skp1 has not been demonstrated. To address this issue, and to investigate the generality of O2 regulation of development, we turned to a previ ously described submerged development model in which terminal cell differentiation depends on high at mospheric O2.

The wider range of O2 concentra tions presented to cells in this setting may facilitate analysis of the dependence of Skp1 hydroxylation on O2, and absence of the morphogenetic movements of cul mination might reveal later developmental steps that are dependent on Skp1 and its modifications. In a static adaptation of the previous shaking cultures, we now observed that terminal cell differentiation occurs in a novel radi ally symmetrical fashion in multicellular cyst like struc tures. Under these conditions, we find that O2 is apparently rate limiting for Skp1 hydroxylation, and that cyst formation and terminal spore differentiation that re quire high O2 also

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