“IFN alpha exerts potent inhibitory activities against malignant melanoma cells in vitro and in vivo, but the mechanisms by which it generates its antitumor effects remain unknown. We examined the effects of interferon alpha(IFN alpha) on the expression
of human members of the Schlafen (SLFN) family of genes, a group of cell cycle regulators that mediate growth-inhibitory responses. Using quantitative RT-real time PCR, we found detectable basal expression of all the different human SLFN genes examined (SLFN5, SLFN11, SLFN12, SLFN13, and SLFN14), in malignant melanoma cells and primary normal human melanocytes, but SLFN5 basal expression was suppressed in all analyzed melanoma cell lines. Treatment of melanoma cells with IFN alpha resulted in induction of expression of SLFN5 in Autophagy Compound Library concentration malignant cells, suggesting a potential involvement of this gene in the antitumor effects of IFN alpha. Importantly, stable knockdown of SLFN5 in malignant melanoma cells resulted in increased
anchorage-independent growth, as evidenced by enhanced colony formation in soft agar assays. Moreover, SLFN5 knockdown also resulted in increased invasion in three-dimensional collagen, suggesting a dual role for SLFN5 in the regulation of invasion and anchorage-independent growth of melanoma cells. Altogether, our findings suggest an important role for the SLFN family of proteins in the generation of
the anti-melanoma effects of IFN alpha and for the first time directly implicate a member of the human SLFN family in the regulation of cell invasion.”
“A newly isolated Ganetespib datasheet thermotolerant ethanologenic yeast strain, Issatchenkia orientalis IPE 100, was able to produce ethanol with a theoretical yield of 85% per g of glucose at 42 degrees C. Ethanol production was inhibited by furfural, hydroxymethylfurfural and vanillin concentrations above 5.56 g L(-1), 7.81 g L(-1), and learn more 3.17 g L(-1), respectively, but the strain was able to produce ethanol from enzymatically hydrolyzed steam-exploded cornstalk with 93.8% of theoretical yield and 0.91 g L(-1) h(-1) of productivity at 42 degrees C. Therefore, I. orientalis IPE 100 is a potential candidate for commercial lignocelluloses-to-ethanol production. (C) 2011 Elsevier Ltd. All rights reserved.”
“Background and Objective: Auditory neuropathy (AN) is a term used to describe specific hearing disorder with abnormal auditory neural responses in the presence of normal cochlear function. Post Auricular Muscles Response (PAMR) is a sound evoked myogenic potential could be useful for hearing evaluation. Though the exact pathway of the PAMR is not known in human, however it is a brainstem reflex. Theoretically, the absent acoustic reflex in AN may be indicative for abnormal PAMR as well. The aim of this study is to investigate the PAMR in patients with AN.