For the HPTLC method by LLE as shown in Figure 2, there is no any interference of the biological matrix in the quantitation of CEFPO and AMBRO. Sensitivity of the method is defined as the lowest concentration that can be measured with an acceptable limit of accuracy and precision which is lower than 20%.[10] The accuracy and precision at a lower limit of quantitation (LLOQ) analyzed therefore by using five replicate (n = 5) of the sample at the LLOQ concentration. The accuracy is determined by %RE at this LLOQ concentration. The lower limit of quantitation was found to be 500 ng/spot and 1000 ng/ spot with %RE = 2.382, 0.198 and %RSD = 17.28, 5.96 for CEFPO and AMBRO within acceptable limits. Analysis speed In case of HPTLC 18 spots can be applied on one plate, so it is less time consuming.

Stability In bench top stability, the low and high QC samples were thawed and allowed to remain at room temperature for 12 h. A comparison of the results for the QC sample (low and high) with freshly prepared stock solution showed that there was no significant difference between response of freshly prepared solution and a sample of CEFPO and AMBRO after 12 h. Freeze�Cthaw stability was determined after two freeze�Cthaw cycles for three replicate of low and high QC samples. The samples were stored at �C20��C temperature for 24 h and then thawed at room temperature. No significant difference between the freeze�Cthaw sample and freshly prepared sample was observed. The result of stability experiments shows that no significant degradation occurred at ambient temperature for 48 h for post preparative stability.

Results of stability for the HPTLC method are shown in Table 3. Table 3 Stability study of cefpodoxime proxetil and ambroxol hydrochloride in human plasma CONCLUSION The proposed HPTLC method for the estimation of CEFPO and AMBRO in human plasma is selective and sensitive. The method is economical and faster than earlier published methods. In future, these methods Batimastat can be used for bioequivalence study. ACKNOWLEDGMENTS The authors are thankful to the Management and Principal, Dr. Rajendra S. Bhambar, M. G. V.’s Pharmacy College, Nashik, for providing the necessary facilities for the research work. The authors are also thankful to Arpan Blood Bank, Nashik, for providing human plasma and to Blue Cross Laboratories Ltd. Ambad Nashik, India, for providing CEFPO and AMBRO and to Kirti Pharmachem., Sinner, Maharashtra, India, for providing paracetamol as a gift sample for the research work. Footnotes Source of Support: Nil. Conflict of Interest: None declared.
Telmisartan (TELM) is chemically known as 4��-[(1,4��-dimethyl-2-propyl [2,6��-bi-1H-benzimidazol]-1��-yl) methyl] [1,1��-biphenyl]-2-carboxylic acid.