avium subsp. avium M. avium complex** 2   D M. kansasii type 1 M. kansasii M. kansasii 6   D M. kansasii type 2 M. kansasii M. kansasii 1   D M. kansasii type 6 M. kansasii M. kansasii 1   D M. triviale type 1 M. triviale M. triviale 1   F M. malmoense type 1 M. malmoense M. malmoense 2   F M. szulgai type 1 M. szulgai M. szulgai 1   F M. interjectum type 1 M. interjectum M. interjectum 1   G M. Erismodegib research buy intracellulare type 1 M. intracellulare M. avium complex** 14   G M. gordonae type 1 M. gordonae M. gordonae 6   G M. gordonae type 2 M. gordonae M. gordonae 1   G M. gordonae type 5 M. gordonae M. gordonae 1   Total       361   * M. peregrinum was identified as M. fortuitum by a conventional biochemical method. **M. avium subsp.

avium and M. intracellulare were identified as M. avium complex by a conventional biochemical method. Discordant results from

rpoB DPRA and Selleckchem CP690550 hsp65 PRA There were 15 isolates (8.6%) of NTM with discordant results with rpoB DPRA and hsp65 PRA (Table 2). The two isolates, Mycobacterial species (A group) and M. flavescens (A group) identified by 16 S rDNA sequencing represented new patterns not available in the hsp65 PRA databases and might be new sub-types in hsp65 PRA. For Mycobacterial species, 16 S rDNA sequencing did not confirm the identity of the isolate but conventional biochemical identification showed it was M. mucogenicum. Table 2 Fifteen isolates of NTM species with discordant results from rpo B RFLP, hsp65 RFLP patterns, Reverse transcriptase 16 S rDNA sequence and conventional biochemical identification No rpoB RFLP pattern hsp65 RFLP pattern 16 S rDNA sequence Conventional Selleck AZD1390 biochemical identification 1 A BstEII : 242.8*, 214.0, 0 M. flavescens M. flavescens     HaeIII: 130.9, 140, 90.4, 49.7, 41.5, 37.1     2 A BstEII :456.3, 0, 0 Mycobacterial species M. mucogenicum     HaeIII:192.6, 90.4, 82.0     3 D M. scrofulaceum type 1 M. scrofulaceum M. scrofulaceum 4 G M. simiae type 5 M. simiae M. simiae 5 G M. simiae type 5 M. simiae M. simiae 6 F M. intracellulare type 3 M. intracellulare

M. avium complex** 7 F M. gordonae type 3 M. gordonae M. gordonae 8 F M. gordonae type 3 M. gordonae M. gordonae 9 F M. gordonae type 3 M. gordonae M. gordonae 10 F M. gordonae type 3 M. gordonae M. gordonae 11 F M. gordonae type 3 M. gordonae M. gordonae 12 F M. gordonae type 3 M. gordonae M. gordonae 13 F M. gordonae type 3 M. gordonae M. gordonae 14 F M. gordonae type 4 M. gordonae M. gordonae 15 F M. gordonae type 4 M. gordonae M. gordonae *fragment size by CE. ** M. avium subsp. avium and M. intracellulare were identified as M. avium complex by a conventional biochemical method. Development of a species identification algorithm The results in Tables 1 and 2 and the mycobacterial identification flow chart (Figure 1) were used to develop a species identification algorithm by combining rpoB duplex PCR [10] and hsp65 PRA [3] using the most common 74 patterns of 40 species in Table 3. In this algorithm (Table 3), we added M.