8 ± 7.5). All procedures were in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the National Institute for Physiological Sciences, Okazaki, Japan. All subjects gave their written SCH727965 datasheet consent prior to participation. Two experiments Experiments were conducted in a dimly lit, magnetically shielded room. The subjects were seated with their head firmly fixed using a whole-head neuromagnetometer. Experiments consisted of two parts; recording of MRCFs during finger movements of the right hand,

and recording of the somatosensory evoked magnetic fields Inhibitors,research,lifescience,medical (SEFs) following median nerve stimulation of the same side. The two experiments were conducted in this order on the Inhibitors,research,lifescience,medical same day. MRCF experiment Movement For movement experiments, the forearm was placed comfortably on a table, with the elbow joint flexed 70°. The forearm was pronated to bring the hand into a palm-down position, with all fingers and the thumb flexed naturally. The subjects performed voluntary, impulsive extension with the right index finger at the metacarpophalangeal (MP) joint, followed by immediate return of the finger to the initial resting position. A small plastic plate (1 cm height, 2.0 cm long, 0.3 mm thick)

fixed vertically to the tip end Inhibitors,research,lifescience,medical of the index finger was placed into a vertical trench (0.6 mm width, 5 cm long in vertical). Cut ends of optical fibers were placed at the same height on both sides of the Inhibitors,research,lifescience,medical inner walls of the trench to face each other, such that the light signal was transmittable in open space. When the finger was resting, the plastic plate occluded the switch circuit. Once the finger extended (or moved upward), light was transmitted to switch on the circuit and generate a square pulse, which was used as a trigger signal of averaging in the off-line analyses. The other pair of optical fibers Inhibitors,research,lifescience,medical was placed at a height comparable to the fully extended position of the finger, and the corresponding switch circuit generated a trigger pulse when the finger plate occluded the light transmission between these optical fibers. When the index finger was fully extended (0°), therefore, the subject could

see the light projected on the plate as a small dot (diameter 5 mm). Cytidine deaminase The subjects were asked to generate an impulsive force to extend their index finger by an amount sufficient to project the light dot on the center of finger plate, and then immediately relax their finger or hand muscles without activation of antagonist muscles. We encouraged the subjects to move the finger in a self-paced manner with an intertrial interval longer than 5 sec. The subjects were asked to keep their gaze on the vertical trench and to minimize the number of blinks and saccadic eye movements across the recording period. To prevent movement overshoot or undershoot, the subjects were allowed a number of practice trials. The recording period was 20 min, in which two rest periods of 1 minute were inserted among three 6-min trial sessions.