14)     + Vancomycin 30 μg 24.75 (0.04)     + Bacitracin 10 μg 0 (0) +     Novobiocin 30 μg 34.5 (0.07)     + Kanamycin 30 μg 24.15 (0.21)     + Neomycin 30 μg 20 (0)   +   Polymixin B 300 Units 0 (0) +     Oxytetracycline 30 μg 21 (0)     + Cefamandole 30 μg 12 (0) +     For all experiments coefficient of variation was ≤5 %. Results (zone

of inhibition) are expressed as mean (SD). R, resistant; I, intermediate; S, susceptible. β-galactosidase activity The isolate Kp10 (P. acidilactici) produced Angiogenesis inhibitor blue/green SB203580 colonies on M17 agar supplemented with X-gal and IPTG, which confirmed the ability to secrete β-galactosidase. Tolerance to bile salts The ability of Kp10 (P. acidilactici) to tolerate bile salts is shown in Figure 3. Percent survival was >95% after 1 h incubation but was reduced to 89% after 4 h. Figure 3 Tolerance of the isolate Kp10 ( P. acidilactici ) to acidic conditions and bile salts. Results are expressed as mean and standard deviation;

tests were performed in triplicate. Tolerance to low pH The ability of Kp10 (P. acidilactici) to tolerate acidic conditions is shown in Figure 3. Percent survival at pH 3 was >97% after 1 to 3 h incubation. Effect of pH and enzymes on BLIS activity The effect of pH on Kp10 BLIS activity is shown in Table 6. BLIS was stable after a 1-h incubation at pH 2 to 9, but activity was considerably reduced at pH 10 and not detectable at pH 11. The effect of various enzymes on BLIS activity is shown in Table 7. Kp10 BLIS activity this website was retained in the presence of pepsin, α-amylase, and catalase but not in the presence of proteinase K or trypsin. Table 6 Effect of pH on BLIS activity pH BLIS activity (AU/ mL) Control 6,853 2 6,853 3 6,853 4 6,853 5 6,853 6 6,853 7 6,853 8 6,853

9 6,853 10 1,593 11 ND ND, not detected. Table 7 Effect of enzymes on BLIS activity Enzyme BLIS activity (AU/mL) Control 6,853 Proteinase K ND Trypsin ND Pepsin 6,853 α-Amylase 6,853 Catalase 6,853 ND, not detected. Discussion and conclusions In recent years much attention has focused on bacteriocin-producing LAB isolated from Thiamine-diphosphate kinase various sources, because bacteriocins are considered safe as food biopreservatives and can be degraded by gastrointestinal proteases [9]. However, LAB species present in traditional foods of Southeast Asian countries have not been widely studied [10]. In this study, 11 LAB strains isolated from traditional fermented milk products and cocoa beans from rural areas of Malaysia and Iran were found to produce antimicrobial substances. These LAB isolates were characterized, and two of the strains (Kp8 and Kp10) produced substances active against Listeria monocytogenes (888.56 AU/mL). Phenotypic characterization based on sugar fermentation reveals biochemical properties of the microorganisms [11] but may not always provide a strong basis for LAB identification [12].