ABA significantly inhibited the synthesis of ATP at 10 μM and reached a maximum effect at 15 μM. The ANT is an important component of the mitochondrial machinery of ATP synthesis because of its intrinsic adenine nucleotide translocase activity. ANT participates in both pathological (mitochondrial permeability

transition selleck compound formation/regulation and cell death) and physiological (adenine nucleotide exchange) mitochondrial events, making it a prime target for drug-induced toxicity (Oliveira and Wallace, 2006). To demonstrate ABA-induced inhibition of ATPase and/or ANT, we evaluated its effects in the activity of ATPase using intact-uncoupled and freeze–thawing-disrupted mitochondria with an excess of ATP, a condition that drives the enzyme to operate in the reverse direction, hydrolyzing ATP (Bracht et al., 2003), and also in the ADP-induced depolarization of Δψ. We saw more significant stimulation of ATPase activity in intact-uncoupled mitochondria than in disrupted mitochondria, which taken together with the observed inhibition of ADP-induced depolarization of Δψ indicates that abamectin more specifically inhibits ANT than FoF1-ATPase.

Adriamycin In conclusion, the present study shows that ABA perturbs the mitochondrial bioenergetics through different mechanisms and that its effect on the adenine nucleotide translocator (ANT) is more potent than on FoF1-ATPase. These effects constitute a potential mechanism for ABA toxicity in liver cells, which could contribute to the toxicological effects of ABA described in animals and human. The authors declare that there are no conflicts of interest. This work was supported by grants from Fundação de Amparo

à Pesquisa do Estado de São Paulo (FAPESP). Results will be presented by Juliana Carla Castanha click here Zanoli to the Faculdade de Medicina Veterinária de Araçatuba, Universidade Estadual Paulista “Júlio de Mesquita Filho”, in partial fulfillment of the requirements for the Master degree in Ciência Animal. “
“Phthalocyanines (PCs) are macrocyclic complexes whose π systems (bonds in which the atomic orbitals overlap in parallel, forming an electron density cloud above and below the internuclear axis) (Graham Solomons and Fryhle, 2001 and Pine et al., 1982) are delocalized over an arrangement of conjugated carbon and nitrogen atoms, providing for their unique chemical and physical properties (Fig. 1) (Leznoff and Lever, 2004 and Mckeown, 1998). Due to the significance of the structural component of the π system in PCs, studies on the nature of the π system and attempts to modulate it have been intensively investigated (Day et al., 1975 and Svetlana et al., 1996). Many of the properties of PCs are highly dependent on the extent of intermolecular π–π stacking interactions between the planar faces of the macrocycles.